ABSTRACT
Objective To observe the effects of short-term blood flow restriction (BFR) based on limb linkage training on cardiopulmonary endurance, lower limb muscle endurance and balance function of healthy people. Methods From December, 2019 to March, 2020, 20 healthy young people were randomly divided into control group (n = 10) and experimental group (n = 10). They accepted limb linkage training with the loading of anaerobic threshold (AT) according to the results of cardiopulmonary exercise test (CPET), 20 minutes a time, three times a week, for two weeks; while the experimental group wore 250 mmHg blood pressure training belt at the root of both thighs; the control group wore blood pressure training belt with no pressure. They were assessed with CPET, 6-minute walking test, wall squatting, seat test and functional reach test before and after training. Results After training, all the indexes improved in both groups (|t| > 2.321, P < 0.05), except functional reach test, the results of wall squatting and seat test were better in the experimental group than in the control group (t > 2.660, P < 0.05). Conclusion Limb linkage training combined with lower extremity BFR could further improve lower limb muscle endurance.
ABSTRACT
Objective To observe the effects of short-term blood flow restriction (BFR) based on limb linkage training on cardiopulmonary endurance, lower limb muscle endurance and balance function of healthy people. Methods From December, 2019 to March, 2020, 20 healthy young people were randomly divided into control group (n = 10) and experimental group (n = 10). They accepted limb linkage training with the loading of anaerobic threshold (AT) according to the results of cardiopulmonary exercise test (CPET), 20 minutes a time, three times a week, for two weeks; while the experimental group wore 250 mmHg blood pressure training belt at the root of both thighs; the control group wore blood pressure training belt with no pressure. They were assessed with CPET, 6-minute walking test, wall squatting, seat test and functional reach test before and after training. Results After training, all the indexes improved in both groups (|t| > 2.321, P < 0.05), except functional reach test, the results of wall squatting and seat test were better in the experimental group than in the control group (t > 2.660, P < 0.05). Conclusion Limb linkage training combined with lower extremity BFR could further improve lower limb muscle endurance.
ABSTRACT
Objective:To independently develop a power control system of ultrasonic scalpel so as to reduce the energy consumption and maintain the normal temperature of ultrasonic scalpel.Methods:In this paper, the model of equivalent circuit of ultrasonic transducer nearby syntony was built up, and the hardware control system of ultrasonic scalpel based on digital signal processing (DSP) was designed.Results:Through testing the circuit and work performance of power control system, the series of parameters such as effective value and so on which were produced by this system could adjust frequency of power source in time and attain anticipative functional indicator, and it took the ultrasonic scalpel to work in syntonic situation.Conclusion:The tested indicators of power control system of ultrasonic scalpel based on the kernel design of DSP can attain anticipative requirement, and can take this system to work in syntonic situation.
ABSTRACT
<p><b>BACKGROUND</b>There are few reports of a biological role for glycosyltransferases in the infiltration of osteoarthritic synovitis. The aim of this research was to investigate the expression and cellular location of β-1,4-galactosyltransferase I (β-1,4-GalT-I) in a surgically-induced rat model of knee osteoarthritis (OA), and explore the role of β-1,4-GalT-I in the pathogenesis of OA.</p><p><b>METHODS</b>Male Sprague-Dawley rats were randomly divided into three groups: OA group, sham group and normal group. The model of OA was established in the right knees of rats by anterior cruciate ligament transaction (ACLT) with partial medial meniscectomy. Fibroblast-like synoviocytes (FLSs) obtained from normal rat synovial tissue were cultured. The expression of β-1,4-GalT-I mRNA in the synovial tissue, articular cartilage and FLSs treated with tumor necrosis factor-α (TNF-α) were assayed by real-time PCR. Western-blotting and immunohistochemisty were used to observe the expression of β-1,4-GalT-I at the protein level. Double immunofluorescent staining was used to define the location of the β-1,4-GalT-I with macrophage-like synoviocytes, FLSs, neutrophils, and TNF-α in the OA synovium. The alteration of TNF-α in FLSs which were treated with lipopolysaccharide (LPS) and β-1,4-GalT-I-Ab were detected by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The mRNA and protein expression of β-1,4-GalT-I increased in synovial tissue of the OA group compared with the normal and sham groups at two and four weeks after the surgery, however, no significant difference appeared in the articular cartilage. Immunohistochemistry also indicated that the β-1,4-GalT-I expression in OA synovium at four weeks after surgery increased sharply compared with the control group. β-1,4-GalT-I co-localized with macrophage-like synoviocytes, FLSs, neutrophils and TNF-α in rat OA synovitis. Moreover, in vitro β-1,4-GalT-I mRNA in FLSs was affected in a dose- and time-dependent manner in response to TNF-α stimulation. ELISA revealed that the expression of TNF-α was attenuated in FLSs in vitro when treated with anti β-1,4-GalT-I antibody.</p><p><b>CONCLUSION</b>β-1,4-GalT-I may play an important role in the inflammation process of rat OA synovial tissue which would provide the foundation for further researching into the concrete mechanism of β-1,4-GalT-I in OA synovitis.</p>